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https://hdl.handle.net/1889/778
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DC Field | Value | Language |
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dc.contributor.advisor | Dall'Asta, Valeria | - |
dc.contributor.author | Barilli, Amelia | - |
dc.date.accessioned | 2008-06-03T08:16:25Z | - |
dc.date.available | 2008-06-03T08:16:25Z | - |
dc.date.issued | 2008-03-04 | - |
dc.identifier.uri | http://hdl.handle.net/1889/778 | - |
dc.description.abstract | The research concerns the characterization of the pathways responsible for the recruitment of L-arginine, the obliged substrate for nitric oxide biosynthesis, in human cells. Endothelial cells and monocytes/macrophages have been employed, as the cell types more directly linked to NO pathway. As for human endothelium, only system y+ is involved in the inflammatory response: both TNFα and rapamycin, an mTOR inhibitor employed in clinical angioplasty, lead to a massive increase of CAT-mediated arginine influx and to the activation of endothelial cells; however, both compounds are ineffective in stimulating the synthesis of NO, and even diminish the expression of eNOS mRNA and protein. Rapamycin also causes a significant loss of cell viability and function, thus confirming the adverse effects of the drug observed in vivo. Among cells of the human monocyte/macrophage lineage, differences in the modulation of arginine transport by cytokines have emerged: IFNγ stimulates system y+L activity in blood monocytes, while alveolar macrophages are insensitive to inflammatory stimuli; also in these models, the production of NO is undetectable even in the presence of inflammatory cytokines. The supposed co-induction of arginine transport and NO biosynthesis under inflammatory conditions may thus not be valid for human cells, although plausible in animal models. | en |
dc.language.iso | Inglese | en |
dc.publisher | Università degli Studi di Parma, Dipartimento di Medicina Sperimentale | en |
dc.relation.ispartofseries | Dottorato di ricerca in Biologia e patologia molecolare | en |
dc.rights | © Amelia Barilli, 2008 | en |
dc.subject | Arginine transport | en |
dc.subject | Nitric oxide | en |
dc.subject | Human endothelium | en |
dc.subject | Human monocytes/macrophages | en |
dc.title | Arginine transport and nitric oxide production: role in the inflammatory response | en |
dc.type | Doctoral thesis | en |
dc.subject.miur | MED/04 | en |
dc.description.fulltext | open | en |
Appears in Collections: | Scienze biomediche, biotecnologiche e traslazionali. Tesi di dottorato |
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File | Description | Size | Format | |
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PhD thesis.pdf | Tesi di Dottorato | 1.93 MB | Adobe PDF | View/Open |
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