Innovative mass spectrometry-based analytical strategies in proteomics

Abstract

This PhD thesis was divided in four main sections. In particular, the first section is composed by fourth chapters showing the main components of a mass spectrometer such as sources, analyzers and detectors followed by a brief introduction of mass spectrometry-based proteomics. In the second chapter, the proteomics analysis of platelet-derived microparticles under different agonist stimulations has been described. In the third chapter, the PTMs analysis of platelet-derived microparticles has been presented. In particular, phosphorylation and glycosylation have been intensely studied. In the fourth chapter, different sample treatment methods for the simultaneous quantification of caseins and ovalbumin in red wine have been investigated. This thesis has presented an overview of mass spectrometry-based analytical strategies in proteomics and has shown that mass spectrometry has become essential in the protein analysis in both biological and food samples.