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https://hdl.handle.net/1889/2293
Full metadata record
DC Field | Value | Language |
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dc.contributor.advisor | Sansone, Francesco | - |
dc.contributor.advisor | Casnati, Alessandro | - |
dc.contributor.author | Lomazzi, Michela | - |
dc.date.accessioned | 2013-07-17T09:13:10Z | - |
dc.date.available | 2013-07-17T09:13:10Z | - |
dc.date.issued | 2013-03 | - |
dc.identifier.uri | http://hdl.handle.net/1889/2293 | - |
dc.description.abstract | At the basis of gene therapy stays as fundamental process the delivery of proper nucleic acids into the cell nucleus. This process needs efficient and safe vectors able to condense and protect DNA filaments, masking their phosphate negative charges and thus promoting membrane crossing. Due to their intrinsic properties, Cell Penetrating Peptides, and in particular the arginine-rich ones, have attracted attention in this field as adjuvant for DNA cargo in cell membrane crossing. The work reported in this PhD thesis concerns the synthesis of new potential non-viral vectors designed and built on macrocyclic scaffolds with well-defined cationic and lipophilic domains. They are functionalized with arginines, lysines, guanidinylated amino acids or ammonium ions. Their ability in DNA binding and condensation and their transfection properties in vitro were investigated also looking for establishing relationships between activity and structural features. | it |
dc.language.iso | Inglese | it |
dc.publisher | Università degli Studi di Parma. Dipartimento di Chimica | it |
dc.relation.ispartofseries | Dottorato di ricerca in Scienze Chimiche | it |
dc.rights | © Michela Lomazzi, 2013 | it |
dc.subject | Cell transfection | it |
dc.subject | Gene delivery | it |
dc.subject | Calixarenes | it |
dc.subject | β-cyclodextrins | it |
dc.subject | Non-viral vectors | it |
dc.subject | Basic amino acids | it |
dc.subject | Arginine | it |
dc.title | Macrocyclic amphiphilic non-viral vectors for gene delivery | it |
dc.type | Doctoral thesis | it |
dc.subject.miur | CHIM/06 | it |
Appears in Collections: | Scienze chimiche. Tesi di dottorato |
Files in This Item:
File | Description | Size | Format | |
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frontesizio.pdf | 81.25 kB | Adobe PDF | View/Open | |
CAP 1_def.pdf | 1.2 MB | Adobe PDF | View/Open | |
Cap2_ def.pdf | 10.1 MB | Adobe PDF | View/Open | |
Cap3_def.pdf | 6.47 MB | Adobe PDF | View/Open | |
Cap4_def.pdf | 4.28 MB | Adobe PDF | View/Open | |
Cap 5_def.pdf | 4.51 MB | Adobe PDF | View/Open |
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